Protective effect of epigallocatechin gallate in murine water-immersion stress model of chronic fatigue syndrome

Basic Clin Pharmacol Toxicol. 2010 Jun;106(6):490-6. doi: 10.1111/j.1742-7843.2009.00525.x. Epub 2010 Jan 18.


Chronic fatigue syndrome (CFS) is a specific clinical condition that characterizes unexplained disabling fatigue. In the present study, chronic fatigue was produced in mice by subjecting them to forced swim inside a rectangular jar of specific dimensions for 6 min. daily for 15 days. Epigallocatechin gallate (EGCG; 25, 50 and 100 mg/kg, p.o.) was administered daily 30 min. before forced swim session. Immobility period and post-swim fatigue was assessed on alternate days. On the 16th day, after assessment of various behavioural parameters, mice were killed to harvest the brain, spleen and thymus. There was significant increase in oxidative-nitrosative stress and tumour necrosis factor-alpha levels in the brain of mice subjected to water-immersion stress as compared with naive group. These behavioural and biochemical alterations were restored after chronic treatment with EGCG. The present study points out that EGCG could be of therapeutic potential in the treatment of chronic fatigue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Brain / metabolism
  • Catechin / administration & dosage
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Fatigue Syndrome, Chronic / drug therapy*
  • Fatigue Syndrome, Chronic / physiopathology
  • Mice
  • Oxidative Stress / drug effects
  • Spleen / metabolism
  • Swimming
  • Thymus Gland / metabolism
  • Tumor Necrosis Factor-alpha / metabolism


  • Antioxidants
  • Tumor Necrosis Factor-alpha
  • Catechin
  • epigallocatechin gallate