Resveratrol, a red wine polyphenol, attenuates lipopolysaccharide-induced oxidative stress in rat liver

Ecotoxicol Environ Saf. 2010 Jul;73(5):1078-83. doi: 10.1016/j.ecoenv.2009.12.031. Epub 2010 Jan 20.

Abstract

Lipopolysaccharide (LPS) is a glycolipid component of the cell wall of gram-negative bacteria inducing deleterious effects on several organs including the liver and eventually leading to septic shock and death. Endotoxemia-induced hepatotoxicity is characterized by disturbed intracellular redox balance, excessive reactive oxygen species (ROS) accumulation inducing DNA, proteins and membrane lipid damages. Resveratrol (trans-3,5,4' trihydroxystilbene) is a phytoalexin polyphenol exhibiting antioxidant and anti-inflammatory properties. In this study, we investigated the effect of subacute pre-treatment with this natural compound on LPS-induced hepatotoxicity in rat. Resveratrol counteracted LPS-induced lipoperoxidation and depletion of antioxidant enzyme activities as superoxide dismutase (SOD) and catalase (CAT) but slightly glutathione peroxidase (GPx) activity. The polyphenol also abrogated LPS-induced liver and plasma nitric oxide (NO) elevation and attenuated endotoxemia-induced hepatic tissue injury. Importantly resveratrol treatment abolished LPS-induced iron sequestration from plasma to liver compartment. Our data suggest that resveratrol is capable of alleviating LPS-induced hepatotoxicity and that its mode of action may involve differential iron compartmentalization via iron shuttling proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Iron / metabolism
  • Lipid Peroxidation / drug effects
  • Lipopolysaccharides / toxicity*
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Nitric Oxide / metabolism
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Wistar
  • Resveratrol
  • Stilbenes / pharmacology*

Substances

  • Antioxidants
  • Lipopolysaccharides
  • Stilbenes
  • Nitric Oxide
  • Iron
  • Resveratrol