Development and characterisation of a collagen nano-hydroxyapatite composite scaffold for bone tissue engineering

J Mater Sci Mater Med. 2010 Aug;21(8):2293-8. doi: 10.1007/s10856-009-3964-1. Epub 2009 Dec 20.


Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (>98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- 1.70 kPa vs. 0.30 +/- 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Bone Regeneration / physiology
  • Bone Substitutes / chemical synthesis
  • Bone Substitutes / chemistry
  • Bone Substitutes / pharmacology
  • Bone and Bones / cytology
  • Bone and Bones / physiology*
  • Cattle
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Collagen / chemical synthesis*
  • Collagen / chemistry
  • Collagen / pharmacology
  • Durapatite / chemical synthesis*
  • Durapatite / chemistry
  • Durapatite / pharmacology
  • Guided Tissue Regeneration / methods
  • Mice
  • Spectroscopy, Fourier Transform Infrared
  • Tissue Engineering / methods*
  • Tissue Scaffolds* / chemistry


  • Biocompatible Materials
  • Bone Substitutes
  • nano-hydroxyapatite-collagen
  • Collagen
  • Durapatite