Ganodermasides A and B, two novel anti-aging ergosterols from spores of a medicinal mushroom Ganoderma lucidum on yeast via UTH1 gene

Bioorg Med Chem. 2010 Feb;18(3):999-1002. doi: 10.1016/j.bmc.2009.12.070. Epub 2010 Jan 6.

Abstract

Two novel ergosterol derivatives, ganodermasides A and B, hydroxylated at C-15 were isolated from the methanol extract of spores of a medicinal mushroom, Ganoderma lucidum, showed to extend the replicative life span of Saccharomyces cerevisiae, a yeast of K6001 strain. The stereostructures of ganodermasides A and B were determined based on the spectroscopic analysis and comparison of spectroscopic data. These new sterols have a 4, 6, 8(14), 22-tetraene-3-one unit with a unique hydroxylation at C-15. The anti-aging activity of these compounds on yeast is comparable to a well-known substance, resveratrol. Based on results of the investigation of the mechanism of biological activity, ganodermasides A and B regulated UTH1 expression in order to extend the replicative life span of yeast.

MeSH terms

  • Aging / drug effects
  • Drugs, Chinese Herbal / chemistry
  • Drugs, Chinese Herbal / isolation & purification
  • Drugs, Chinese Herbal / pharmacology
  • Ergosterol / chemistry
  • Ergosterol / isolation & purification*
  • Ergosterol / pharmacology*
  • Gene Expression Regulation, Fungal / drug effects
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mitochondrial Proteins / genetics*
  • Mitochondrial Proteins / metabolism
  • Molecular Structure
  • Reishi / chemistry*
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Spores, Fungal / chemistry*

Substances

  • Drugs, Chinese Herbal
  • Heat-Shock Proteins
  • Membrane Proteins
  • Mitochondrial Proteins
  • Saccharomyces cerevisiae Proteins
  • UTH1 protein, S cerevisiae
  • Ergosterol