Genomic characterization of a novel human papillomavirus (HPV-117) with a high viral load in a persisting wart

Virology. 2010 Mar 30;399(1):129-133. doi: 10.1016/j.virol.2009.12.023. Epub 2010 Jan 22.


Warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. Human papillomaviruses (HPV) are considered the causal agents for the development of such warts. We isolated the novel type HPV-117 from a persisting wart by rolling circle amplification. One hundred eighteen warts from immunocompetent patients (IC) and 49 warts from OTR were analyzed by HPV-117 E6 type-specific PCR. As inferred from a phylogenetic analysis, the new type HPV-117 belonged to alpha-PV species 2, including the most similar types HPV-10 and HPV-94. The general prevalence of HPV-117 in warts was 2% in IC (2/118), and 12% in OTR (6/49). The high viral load in dysplastic cells of a Verruca vulgaris was shown by in situ hybridization. Our results suggest an active role of the novel type in the development of cutaneous warts of OTR.

MeSH terms

  • Adult
  • Genome, Viral / genetics*
  • Humans
  • Immunocompromised Host
  • In Situ Hybridization
  • Male
  • Molecular Sequence Data
  • Papillomaviridae / genetics*
  • Papillomaviridae / pathogenicity
  • Papillomavirus Infections / genetics
  • Papillomavirus Infections / virology*
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Viral Envelope Proteins / genetics
  • Viral Load*
  • Warts / virology*


  • Viral Envelope Proteins

Associated data

  • GENBANK/GQ246950