Proteomics approaches to understand protein phosphorylation in pathway modulation

Curr Opin Plant Biol. 2010 Jun;13(3):280-87. doi: 10.1016/j.pbi.2009.12.008. Epub 2010 Jan 22.


Signaling pathways in all organisms consist of series of phosphorylation and dephosphorylation events that define directionality and allow different levels of feedback-regulation. Mass spectrometry-based proteomic analyses in recent years have led to a proteome-wide identification of thousands of phosphorylation sites in various plant species. Given this magnitude of mostly qualitative information about protein phosphorylation, discovery of specific phosphoproteins with regulatory functions represents a major challenge. In future large-scale experiments, combinations of data-driven modeling strategies based on quantitative data, targeted kinase-substrate screens, and verification in biochemical and genetic experiments are required to specifically spot phosphorylation sites with specific roles in signaling pathway modulation.

Publication types

  • Review

MeSH terms

  • Mass Spectrometry
  • Phosphorylation
  • Plant Proteins / metabolism*
  • Plants / metabolism*
  • Protein Processing, Post-Translational*
  • Proteome / metabolism
  • Proteomics*
  • Signal Transduction


  • Plant Proteins
  • Proteome