Geranylfarnesyl Diphosphate Synthase From Methanosarcina Mazei: Different Role, Different Evolution

Biochem Biophys Res Commun. 2010 Feb 26;393(1):16-20. doi: 10.1016/j.bbrc.2010.01.063. Epub 2010 Jan 25.


The gene of (all-E) geranylfarnesyl diphosphate synthase that is responsible for the biosynthesis of methanophenazine, an electron carrier utilized for methanogenesis, was cloned from a methanogenic archaeon Methanosarcina mazei Gö1. The properties of the recombinant enzyme and the results of phylogenetic analysis suggest that the enzyme is closely related to (all-E) prenyl diphosphate synthases that are responsible for the biosynthesis of respiratory quinones, rather than to the enzymes involved in the biosynthesis of archaeal membrane lipids, including (all-E) geranylfarnesyl diphosphate synthase from a thermophilic archaeon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Evolution, Molecular
  • Farnesyltranstransferase / chemistry
  • Farnesyltranstransferase / genetics
  • Farnesyltranstransferase / metabolism*
  • Methane / biosynthesis*
  • Methanosarcina / enzymology*
  • Molecular Sequence Data
  • Phenazines / metabolism*
  • Phylogeny
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Alignment


  • Phenazines
  • Recombinant Proteins
  • methanophenazine
  • Farnesyltranstransferase
  • Methane