Temporal Changes in Dendritic Cell Subsets, Cross-Priming and Costimulation via CD70 Control CD8(+) T Cell Responses to Influenza

Nat Immunol. 2010 Mar;11(3):216-24. doi: 10.1038/ni.1838. Epub 2010 Jan 24.

Abstract

The question of which dendritic cells (DCs) respond to pulmonary antigens and cross-prime CD8(+) T cells remains controversial. We show here that influenza-specific CD8(+) T cell priming was controlled by different DCs at different times after infection. Whereas early priming was controlled by both CD103(+)CD11b(lo) and CD103(-)CD11b(hi) DCs, CD103(-)CD11b(hi) DCs dominated antigen presentation at the peak of infection. Moreover, CD103(-)CD11b(hi) DCs captured exogenous antigens in the lungs and directly cross-primed CD8(+) T cells in the draining lymph nodes without transferring antigen to CD8alpha(+) DCs. Finally, we show that CD103(-)CD11b(hi) DCs were the only DCs to express CD70 after influenza infection and that CD70 expression on CD103(-)CD11b(hi) DCs licensed them to expand CD8(+) T cell populations responding to both influenza and exogenous ovalbumin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD27 Ligand / immunology*
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / virology
  • Cross-Priming / immunology
  • Dendritic Cells / immunology*
  • Dendritic Cells / virology
  • Flow Cytometry
  • Histocompatibility Antigens Class II / immunology
  • Immunophenotyping
  • Influenza A virus / immunology*
  • Kinetics
  • Lymph Nodes / immunology
  • Lymph Nodes / virology
  • Mice
  • Mice, Inbred BALB C
  • Nucleocapsid Proteins / immunology
  • Orthomyxoviridae Infections / immunology*
  • Orthomyxoviridae Infections / virology

Substances

  • CD27 Ligand
  • Histocompatibility Antigens Class II
  • Nucleocapsid Proteins