High-velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo

FEBS Lett. 1991 Mar 11;280(1):94-6. doi: 10.1016/0014-5793(91)80212-l.

Abstract

Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p chi-Casein-cat, p beta-Casein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.

MeSH terms

  • Animals
  • Chloramphenicol O-Acetyltransferase / genetics*
  • DNA / chemistry
  • In Vitro Techniques
  • Kidney / enzymology*
  • Liver / enzymology*
  • Mammary Glands, Animal / enzymology*
  • Promoter Regions, Genetic
  • Rats
  • Rats, Inbred Strains
  • Transfection
  • Transformation, Genetic

Substances

  • DNA
  • Chloramphenicol O-Acetyltransferase