Comparison of three methods for accurate quantification of hydrogen sulfide during fermentation

Anal Chim Acta. 2010 Feb 15;660(1-2):87-91. doi: 10.1016/j.aca.2009.09.049. Epub 2009 Oct 7.


Two analytical approaches for the rapid measurement of hydrogen sulfide (H(2)S) have been compared to a reference method for their potential application as a rapid procedure for the quantification of H(2)S formed during alcoholic fermentations. In one case, silver nitrate, lead acetate, and mercuric chloride selective detector tubes for the analysis of H(2)S in air were investigated. In the other case, a commercially available kit for the diagnosis of nitrogen starvation in wine fermentations, which is based on the detection of H(2)S, was investigated. Both methods exhibited excellent linearity of response, but the mercuric chloride tube was found to suffer from interferences due to the concomitant presence of mercaptans, which resulted in erroneous H(2)S quantification. A comparative study between the two methods studied and the cadmium hydroxide/methylene blue reference method commonly used to monitor H(2)S indicate that the two new methods displayed better recoveries at low H(2)S concentrations, besides being more rapid and economical. The two new methods were successfully used to quantify production of H(2)S in different grape juice fermentations. The suitability of each method for the study of specific aspects of H(2)S production during fermentation is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium / chemistry
  • Fermentation*
  • Food Analysis / methods*
  • Hydrogen Sulfide / analysis*
  • Mercuric Chloride / chemistry*
  • Organometallic Compounds / chemistry*
  • Silver Nitrate / chemistry*
  • Wine / analysis


  • Organometallic Compounds
  • Cadmium
  • Mercuric Chloride
  • Silver Nitrate
  • lead acetate
  • Hydrogen Sulfide