Comparison of methods for estimating phenolic maturity in grapes: correlation between predicted and obtained parameters

Anal Chim Acta. 2010 Feb 15;660(1-2):127-33. doi: 10.1016/j.aca.2009.10.067. Epub 2009 Nov 10.


With the aim of determining the real predictive ability of three methods for measuring phenolic maturity (Glories, ITV and Cromoenos), representative grapes of Merlot, Cabernet sauvignon, Grenache and Tempranillo were harvested at three different ripening levels. The grapes were vinified by triplicate and were also used for phenolic maturity estimation. After that, color intensity, CIELAB coordinates, anthocyanins by spectrometry and HPLC and total phenolics were analysed in wines and also in the different extracts from the three extraction methods. Statistical analysis of data was carried out to determine the real performance of prediction of the different methods. Glories method predicted reasonably the color intensity, CIELAB coordinates and the concentration of anthocyanins and total phenolics in wine although it needs a lot of time and it requires working carefully. On the other hand, Cromoenos method predicted similarly or even better the color and phenolic composition of wine. This method also presents the advantage of being much faster and easier to apply. In contrast, although the ITV method provided reasonable results for anthocyanins and total phenolic compounds its color prediction was not adequate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / analysis
  • Chromatography, High Pressure Liquid / methods*
  • Phenols / analysis*
  • Phenols / isolation & purification
  • Regression Analysis
  • Vitis / chemistry*
  • Wine / analysis


  • Anthocyanins
  • Phenols