Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5

J Infect Dis. 1991 Apr;163(4):769-72. doi: 10.1093/infdis/163.4.769.


To investigate whether immunization with Escherichia coli J5 boiled cells induces antibodies directed at deep core structures, antibodies against J5 lipopolysaccharide (LPS), Re LPS, and lipid A were measured in the serum of 70 volunteers before and 2 weeks after immunization. To improve the sensitivity and the specificity of ELISA, complexes of core LPS with high-density lipoproteins were used instead of free core LPS as antigens. A median three-fold increase in antibodies directed against J5 LPS was observed, but no significant increase in the antibodies against Re LPS or lipid A was found. Since J5 antiserum did not react with several smooth LPS or with Re LPS and lipid A, cross-reactivity could not be demonstrated. Thus, immunization of volunteers with E. coli J5 produced a modest specific antibody response against J5 LPS. The mechanism of protection previously observed with J5 antiserum remains unclear.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / biosynthesis*
  • Antibodies, Bacterial / blood
  • Bacterial Vaccines / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / immunology*
  • Humans
  • Immune Sera / immunology
  • Immunization*
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / biosynthesis
  • Lipopolysaccharides / immunology*
  • Lipoproteins, HDL / immunology


  • Antibodies, Bacterial
  • Bacterial Vaccines
  • Immune Sera
  • Immunoglobulin G
  • Immunoglobulin M
  • Lipopolysaccharides
  • Lipoproteins, HDL