Immunofluorescent microscopical visualization of trails left by gliding Cryptosporidium parvum sporozoites

J Parasitol. 1991 Apr;77(2):315-7.

Abstract

Cryptosporidium parvum sporozoites that exhibited gliding motility in vitro were examined by immunofluorescence with anticryptosporidial monoclonal antibodies (Mabs) for surface antigen deposition on poly-L-lysine-coated glass microscope slides. The Mabs that revealed trails are specific for an immunodominant 23-kDa antigen previously localized to the sporozoite surface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Protozoan / analysis*
  • Antigens, Surface / analysis
  • Cryptosporidium / immunology
  • Cryptosporidium / physiology*
  • Fluorescent Antibody Technique
  • Movement

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • Antigens, Surface