Detection of transmembrane linkages between immunoglobulin or complement receptors and the neutrophil's cortical microfilaments by resonance energy transfer microscopy

J Mol Biol. 1991 Mar 20;218(2):263-8. doi: 10.1016/0022-2836(91)90709-f.

Abstract

By exploiting the 1/r4 (where r is the separation distance between fluorochromes) dependence of energy transfer between parallel lamellae, we have observed transmembrane energy transfer between membrane receptors and cortical microfilaments. Receptors were labeled with donor- or acceptor-conjugated Fab fragments, whereas microfilaments were labeled with acceptor- or donor-conjugated phalloidin reagents. Energy transfer was imaged by optical microscopy. We report that cell surface receptors can be constitutively unlinked, linked or inducibly linked to microfilaments.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actin Cytoskeleton / metabolism*
  • Amino Acid Sequence
  • Energy Transfer
  • Humans
  • Immunoglobulin Fab Fragments / metabolism*
  • Microscopy / methods
  • Molecular Sequence Data
  • Neutrophils / metabolism*
  • Neutrophils / ultrastructure
  • Receptors, Complement / metabolism*
  • Receptors, Immunologic / metabolism*

Substances

  • Immunoglobulin Fab Fragments
  • Receptors, Complement
  • Receptors, Immunologic