The beta-ketoadipate pathway of Acinetobacter baylyi undergoes carbon catabolite repression, cross-regulation and vertical regulation, and is affected by Crc

Microbiology. 2010 May;156(Pt 5):1313-1322. doi: 10.1099/mic.0.037424-0. Epub 2010 Jan 28.

Abstract

The degradation of many structurally diverse aromatic compounds in Acinetobacter baylyi is accomplished by the beta-ketoadipate pathway. In addition to specific induction of expression by certain aromatic compounds, this pathway is regulated by complex mechanisms at multiple levels, which are the topic of this study. Multiple operons feeding into the beta-ketoadipate pathway are controlled by carbon catabolite repression (CCR) caused by succinate plus acetate. The pathways under study enable the catabolism of benzoate (ben), catechol (catA), cis,cis-muconate (catB,C,I,J,F,D), vanillate (van), hydroxycinnamates (hca), dicarboxylates (dca), salicylate (sal), anthranilate (ant) and benzyl esters (are). For analysis of CCR at the transcriptional level a luciferase reporter gene cassette was introduced into the operons. The Crc (catabolite repression control) protein is involved in repression of all operons (except for catA), as demonstrated by the analysis of respective crc strains. In addition, cross-regulation was demonstrated for the vanA,B, hca and dca operons. The presence of protocatechuate caused transcriptional repression of the vanA,B- and hca-encoded funnelling pathways (vertical regulation). Thus the results presented extend the understanding both of CCR and of the effects of Crc for all aromatic degradative pathways of A. baylyi and increase the number of operons known to be controlled by two additional mechanisms, cross-regulation and vertical regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / genetics
  • Acinetobacter / metabolism*
  • Adipates / metabolism*
  • Bacterial Proteins / metabolism*
  • Carbon / metabolism*
  • Gene Expression Regulation, Bacterial*
  • Gluconates / metabolism
  • Lactic Acid / metabolism
  • Metabolic Networks and Pathways
  • Operon

Substances

  • Adipates
  • Bacterial Proteins
  • Gluconates
  • 3-oxoadipic acid
  • Lactic Acid
  • Carbon
  • gluconic acid