Effect of dietary Polyphenon E and EGCG on lung tumorigenesis in A/J Mice

Pharm Res. 2010 Jun;27(6):1066-71. doi: 10.1007/s11095-010-0056-3. Epub 2010 Jan 29.

Abstract

Purpose: To compare the chemopreventive efficacy of Polyphenon E (Poly E), (-)-epigallocatechin-3-gallate (EGCG) and Polyphenon E without EGCG (Poly E-EGCG) on the development of benzo(a)pyrene (B(a)P)-induced lung tumors in A/J mice.

Methods: Female A/J mice were given a single intraperitoneal injection of B(a)P (100 mg/kg body weight). One week after B(a)P injection, animals received AIN-76A purified powder diet containing 0.975% (wt/wt) EGCG, 0.525% (wt/wt) Poly E-EGCG or 1.5% (wt/wt) Poly E for 24 weeks or control diet with no additives.

Results: Poly E treatment significantly decreased tumor multiplicity by 52% and tumor load by 64%, while EGCG and Poly E-EGCG did not significantly inhibit lung tumor multiplicity. EGCG was more stable in a complex mixture (Poly E) than as a pure compound.

Conclusion: EGCG was ineffective when administered by diet likely due to its instability. Thus, EGCG's efficacy on mice lung tumorigenesis requires the presence of other tea catechins.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anticarcinogenic Agents / therapeutic use*
  • Catechin / administration & dosage
  • Catechin / analogs & derivatives*
  • Catechin / therapeutic use
  • Chemoprevention
  • Female
  • Lung / drug effects*
  • Lung / pathology
  • Lung Neoplasms / diet therapy
  • Lung Neoplasms / pathology
  • Lung Neoplasms / prevention & control*
  • Mice

Substances

  • Anticarcinogenic Agents
  • Catechin
  • epigallocatechin gallate
  • polyphenon E