A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria

Cell Host Microbe. 2010 Jan 21;7(1):25-37. doi: 10.1016/j.chom.2009.12.007.


The functional spectrum of a secretion system is defined by its substrates. Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS). We identified three substrates of this system, proteins Tse1-3 (type six exported 1-3), which are coregulated with the secretory apparatus and secreted under tight posttranslational control. The Tse2 protein was found to be the toxin component of a toxin-immunity system and to arrest the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cells; however, it provided a major growth advantage for P. aeruginosa strains, relative to those lacking immunity, in a manner dependent on cell contact and the H1-T6SS. This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / toxicity
  • Antibiosis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Toxins / metabolism*
  • Bacterial Toxins / toxicity
  • Bacteriophages / genetics
  • Colony Count, Microbial
  • Evolution, Molecular
  • Gene Order
  • Genomic Islands
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / growth & development*
  • Pseudomonas aeruginosa / metabolism*


  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Bacterial Toxins
  • Membrane Transport Proteins