Protective Effects of the Pyrolyzates Derived From Bamboo Against Neuronal Damage and Hematoaggregation

J Ethnopharmacol. 2010 Apr 21;128(3):594-9. doi: 10.1016/j.jep.2010.01.045. Epub 2010 Feb 1.

Abstract

Ethnopharmacological relevance: Bamboo species are thought to be originally from Central China, but are now found in many temperate and semi-tropical regions around the world. Although the extracts from bamboo may have antioxidant activities and anti-inflammatory effects, their exact biological activities have not been elucidated.

Aim of the study: Two biological activities of bamboo-derived pyrolyzates were investigated; the protective effects against N-methyl-d-aspartate (NMDA)-induced cell death in primary cultured cortical neuron and the anti-plasmin effects determined by using fibrin and fibrinogen degradation products (FDPs) assay.

Results: Treatment of neuronal cells with pyrolyzates of Phyllostachys pubescens, Phyllostachys nigra and Phyllostachys bambusoides resulted in restored cell viability when compared to untreated cells in an NMDA-induced neuronal cell death assay. In addition, cortical neurons treated with Phyllostachys pubescens and Phyllostachys nigra showed a reduction of apoptosis following exposure to NMDA, as determined by Hoechst 33342 staining. In addition, Phyllostachys nigra pyrolyzates also exhibited anti-plasmin action in a FDP assay. It is of interest to note that pyrolyzates exhibited activities of NMDA-receptor antagonist and antifebrin (ogen), since a combination of NMDA receptor antagonists, glucocorticosteroids, GABAergic drugs and heparin are useful for treatment in delayed postischemic injury.

Conclusion: Our results indicate that the pyrolyzates derived from bamboo may have anti-apoptotic effects, and can be useful as a supplement for ischemic injury treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Bambusa / metabolism
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Cerebral Cortex / cytology*
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • China
  • D-Aspartic Acid / metabolism
  • D-Aspartic Acid / pharmacology
  • N-Methylaspartate / metabolism
  • N-Methylaspartate / pharmacology*
  • Neurons* / cytology
  • Neurons* / drug effects
  • Neurons* / metabolism
  • Rats
  • Rats, Sprague-Dawley

Substances

  • D-Aspartic Acid
  • N-Methylaspartate