Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

Cornea. 2010 Mar;29(3):321-30. doi: 10.1097/ICO.0b013e3181b1165d.


Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation.

Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed.

Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect.

Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / physiology*
  • Animals
  • Blotting, Western
  • Cattle
  • Cell Line, Transformed
  • Cell Movement*
  • Cell Proliferation*
  • Endothelium, Vascular / cytology*
  • Epithelium, Corneal / enzymology*
  • Genetic Vectors
  • Green Fluorescent Proteins / genetics
  • Matrix Metalloproteinase 14 / physiology*
  • Mice
  • Mice, Knockout
  • Mutagenesis, Site-Directed
  • Plasmids
  • Pulmonary Artery / cytology
  • Retroviridae / genetics
  • Transfection


  • Angiogenesis Inhibitors
  • Mmp14 protein, mouse
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Matrix Metalloproteinase 14