Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Nat Struct Mol Biol. 2010 Feb;17(2):244-7. doi: 10.1038/nsmb.1734. Epub 2010 Jan 31.


Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5' end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Codon, Nonsense*
  • Endoribonucleases / metabolism*
  • Polyribosomes / metabolism*
  • RNA Stability
  • RNA, Messenger / metabolism*
  • Saccharomyces cerevisiae / metabolism*


  • Codon, Nonsense
  • RNA, Messenger
  • mRNA decapping enzymes
  • Endoribonucleases