4-Chlorobenzoyl berbamine induces apoptosis and G2/M cell cycle arrest through the PI3K/Akt and NF-kappaB signal pathway in lymphoma cells

Oncol Rep. 2010 Mar;23(3):709-16. doi: 10.3892/or_00000688.


Berbamine is an herbal compound derived from Berberis amurensis, which is used in Chinese traditional medicine. However, few studies have investigated this anti-tumor effect or the underlying mechanisms of berbamine on lymphoma cells. We investigate the effect, as well as the mechanism of action, of 4-chlorobenzoyl berbamine (BBD9) on Raji, L428, Namalwa and Jurkat lymphoma cells lines. Our findings show that BBD9 inhibits cell proliferation and induces cell apoptosis in lymphoma cell lines as well as G2/M cell cycle arrest through PI3K/Akt and NF-kappaB signaling pathways in a caspase-dependent manner. These results may provide new insights into the treatment of lymphoma.

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Benzylisoquinolines / pharmacology*
  • Cell Division / drug effects*
  • Cell Line, Tumor
  • Cyclin B1 / physiology
  • G2 Phase / drug effects*
  • Humans
  • Lymphoma / drug therapy*
  • Lymphoma / pathology
  • NF-kappa B / physiology*
  • PTEN Phosphohydrolase / physiology
  • Phosphatidylinositol 3-Kinases / physiology*
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / physiology*
  • Proto-Oncogene Proteins c-bcl-2 / physiology
  • Signal Transduction / drug effects*
  • X-Linked Inhibitor of Apoptosis Protein / physiology


  • 4-chlorobenzoylberbamine
  • Antineoplastic Agents
  • Benzylisoquinolines
  • CCNB1 protein, human
  • Cyclin B1
  • NF-kappa B
  • Proto-Oncogene Proteins c-bcl-2
  • X-Linked Inhibitor of Apoptosis Protein
  • XIAP protein, human
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • PTEN Phosphohydrolase
  • PTEN protein, human