Protein-phospholipid interactions in blood clotting

Thromb Res. 2010 Apr;125 Suppl 1:S23-5. doi: 10.1016/j.thromres.2010.01.027. Epub 2010 Feb 4.

Abstract

Most steps of the blood clotting cascade require the assembly of a serine protease with its specific regulatory protein on a suitable phospholipid bilayer. Unfortunately, the molecular details of how blood clotting proteins bind to membrane surfaces remain poorly understood, owing to a dearth of techniques for studying protein-membrane interactions at high resolution. Our laboratories are tackling this question using a combination of approaches, including nanoscale membrane bilayers, solid-state NMR, and large-scale molecular dynamics simulations. These studies are now providing structural insights at atomic resolution into clotting protein-membrane interactions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Algorithms
  • Blood Coagulation*
  • Cell Membrane / metabolism
  • Computer Simulation
  • Factor VIIa / chemistry
  • Humans
  • Magnetic Resonance Spectroscopy / methods
  • Models, Biological
  • Nanotechnology / methods
  • Phospholipids / chemistry*
  • Protein Structure, Tertiary
  • Proteins / chemistry*
  • Serine Proteases / chemistry
  • Thromboplastin / metabolism

Substances

  • Phospholipids
  • Proteins
  • Thromboplastin
  • Serine Proteases
  • Factor VIIa