Study of TNFalpha -308G/A and IL6 -174G/C polymorphisms in type 2 diabetes and obesity risk in the Tunisian population

Clin Biochem. 2010 Apr;43(6):549-52. doi: 10.1016/j.clinbiochem.2010.01.008. Epub 2010 Feb 2.


Objectives: We investigated two genetic markers in pro inflammatory molecules : TNFalpha -308G/A and IL6 -174G/C in order to assess their effect on type 2 diabetes (T2D) and obesity in the Tunisian population.

Design and methods: The study sample includes 228 patients with T2D and 300 healthy controls. Genotyping of IL6 -174G/C (rs1800795) was performed using Automated Dye Terminator Sequencing and of TNFalpha -308G/A (rs1800629) using the LightTyper technology.

Results: SNPs IL6 -174G/C and TNFalpha -308G/A are associated neither with T2D (p=0.89, p=0.34 respectively) nor with risk for overweight (p=0.86, p=0.12 respectively) in Tunisian population. Bonferroni correction showed that the founded association of IL6 -174G/C SNP with T2D susceptibility restricted to overweight patients (p(nominal)=0.03, p(corrected)=0.0033) is likely to be a random result.

Conclusion: SNPs IL6 -174G/C and TNFalpha -308G/A are not major contributors to T2D or obesity risk in our Tunisian population.

MeSH terms

  • Adult
  • Aged
  • Case-Control Studies
  • Diabetes Mellitus, Type 2 / epidemiology
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Genetic Linkage
  • Genetic Predisposition to Disease
  • Genetics, Population
  • Genotype
  • Humans
  • Interleukin-6 / genetics*
  • Male
  • Middle Aged
  • Obesity / epidemiology
  • Obesity / genetics*
  • Polymorphism, Single Nucleotide* / physiology
  • Promoter Regions, Genetic / genetics
  • Risk Factors
  • Tumor Necrosis Factor-alpha / genetics*
  • Tunisia / epidemiology


  • IL6 protein, human
  • Interleukin-6
  • Tumor Necrosis Factor-alpha