Eukaryotic DNA primase. Abortive synthesis of oligoadenylates

FEBS Lett. 1991 Mar 25;280(2):281-3. doi: 10.1016/0014-5793(91)80312-q.


Calf thymus DNA polymerase alpha-primase, human placenta DNA polymerase alpha-primase and human placenta DNA primase synthesized oligoriboadenylates of a preferred length of 2-10 nucleotides and multimeric oligoribonucleotides of a modal length of about 10 monomers on a poly(dT) template. The dimer and trimer were the prevalent products of the polymerization reaction. However, only the oligonucleotides from heptamers to decamers were elongated efficiently by DNA polymerase alpha.

MeSH terms

  • Adenine Nucleotides / metabolism*
  • Adenosine Triphosphate / metabolism
  • DNA Polymerase II / metabolism*
  • DNA Primase
  • Humans
  • Oligoribonucleotides / metabolism*
  • Placenta / enzymology*
  • Poly T / metabolism
  • RNA Nucleotidyltransferases / metabolism*
  • Templates, Genetic


  • Adenine Nucleotides
  • Oligoribonucleotides
  • Poly T
  • 2',5'-oligoadenylate
  • Adenosine Triphosphate
  • DNA Primase
  • RNA Nucleotidyltransferases
  • DNA Polymerase II