Structural Basis for Inhibition of Complement C5 by the SSL7 Protein From Staphylococcus Aureus

Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3681-6. doi: 10.1073/pnas.0910565107. Epub 2010 Feb 4.

Abstract

Staphylococcus aureus secretes the SSL7 protein as part of its immune evasion strategy. The protein binds both complement C5 and IgA, yet it is unclear whether SSL7 cross-links these two proteins and, if so, what purpose this serves the pathogen. We have isolated a stable IgA-SSL7-C5 complex, and our crystal structure of the C5-SSL7 complex confirms that binding to C5 occurs exclusively through the C-terminal beta-grasp domain of SSL7 leaving the OB domain free to interact with IgA. SSL7 interacts with C5 >70 A from the C5a cleavage site without inducing significant conformational changes in C5, and efficient inhibition of convertase cleavage of C5 is shown to be IgA dependent. Inhibition of C5a production and bacteriolysis are all shown to require C5 and IgA binding while inhibition of hemolysis is achieved by the C5 binding SSL7 beta-grasp domain alone. These results provide a conceptual and structural basis for the development of a highly specific complement inhibitor preventing only the formation of the lytic membrane attack complex without affecting the important signaling functions of C5a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Complement C5 / antagonists & inhibitors*
  • Complement C5 / chemistry*
  • Complement C5 / immunology
  • Crystallography, X-Ray
  • Exotoxins / immunology*
  • Humans
  • Mutation
  • Protein Structure, Tertiary
  • Rabbits
  • Staphylococcus aureus / immunology*
  • Staphylococcus aureus / pathogenicity

Substances

  • Complement C5
  • Exotoxins
  • staphylococcal exotoxin 1