Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea

Gene. 1991 Feb 1;98(1):53-60. doi: 10.1016/0378-1119(91)90103-i.

Abstract

Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Bacterial
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial*
  • Gentamicins / pharmacology*
  • Micromonospora / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • DNA, Bacterial
  • Gentamicins

Associated data

  • GENBANK/M38577
  • GENBANK/M38578
  • GENBANK/M38579
  • GENBANK/M38580
  • GENBANK/M38581
  • GENBANK/M38582
  • GENBANK/M55520
  • GENBANK/M55521
  • GENBANK/M74185
  • GENBANK/S75878