Single nucleotide polymorphism genotyping in MMP genes: the 5' nuclease assay

Methods Mol Biol. 2010:622:221-9. doi: 10.1007/978-1-60327-299-5_13.

Abstract

Many molecular genetic studies of human diseases involve determining the genotypes for single nucleotide polymorphisms. This chapter summarises a number of different techniques for the single nucleotide polymorphism genotyping which can be applied to MMP genes. The chapter also provides a protocol and technical notes for the 5'-nuclease assay, one of the most commonly used genotyping techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / genetics
  • Deoxyribonucleases / metabolism*
  • Enzyme Assays / methods*
  • Genotype
  • Humans
  • Matrix Metalloproteinases / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide / genetics*
  • Statistics as Topic

Substances

  • DNA
  • Deoxyribonucleases
  • Matrix Metalloproteinases