HemoHIM enhances the therapeutic efficacy of ionizing radiation treatment in tumor-bearing mice

J Med Food. 2010 Feb;13(1):47-53. doi: 10.1089/jmf.2009.1049.


Although radiotherapy is commonly used for a variety of cancers, radiotherapy alone does not achieve a satisfactory therapeutic outcome. In this study, we examined the possibility that HemoHIM can enhance the anticancer effects of ionizing radiation (IR) in melanoma-bearing mice. The HemoHIM was prepared by adding the ethanol-insoluble fraction to the total water extract of a mixture of three edible herbs-Angelica Radix, Cnidium Rhizoma, and Paeonia Radix. Anticancer effects of HemoHIM were evaluated in melanoma-bearing mice exposed to IR. IR treatment (5 Gy at 7 days after melanoma cell injection) reduced the weight of the solid tumors, and HemoHIM supplementation with IR enhanced the decreases in tumor weight (P < .03). In the melanoma-bearing mice treated with IR, HemoHIM administration also increased the activity of natural killer cells and cytotoxic T cells, although the proportions of these cells in spleen were not different. In addition, HemoHIM administration increased the interleukin-2 and tumor necrosis factor-alpha secretion from lymphocytes stimulated with concanavalin A, which seemed to contribute to the enhanced efficacy of HemoHIM in tumor-bearing mice treated with IR. In conclusion, HemoHIM may be a beneficial supplement during radiotherapy for enhancing the antitumor efficacy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angelica
  • Animals
  • Antineoplastic Agents, Phytogenic / administration & dosage
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antineoplastic Agents, Phytogenic / therapeutic use*
  • Antineoplastic Combined Chemotherapy Protocols
  • Chemotherapy, Adjuvant
  • Cnidium
  • Concanavalin A
  • Female
  • Interleukin-2 / metabolism
  • Killer Cells, Natural / metabolism
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism
  • Magnoliopsida*
  • Melanoma, Experimental / drug therapy*
  • Melanoma, Experimental / metabolism
  • Melanoma, Experimental / radiotherapy*
  • Mice
  • Mice, Inbred C57BL
  • Mitogens
  • Paeonia
  • Phytotherapy*
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Plant Roots
  • Rhizome
  • Spleen / drug effects
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism
  • Tumor Necrosis Factor-alpha / metabolism


  • Antineoplastic Agents, Phytogenic
  • Interleukin-2
  • Mitogens
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Concanavalin A