Transcription Factors Elk-1 and SRF Are Engaged in IL1-dependent Regulation of ZC3H12A Expression

BMC Mol Biol. 2010 Feb 6;11:14. doi: 10.1186/1471-2199-11-14.

Abstract

Background: MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored.

Results: Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter.

Conclusions: We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1beta stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Chromatin Immunoprecipitation
  • Hep G2 Cells
  • Humans
  • Interleukin-1beta / pharmacology*
  • MAP Kinase Signaling System
  • Macrophages / immunology
  • Macrophages / metabolism
  • NF-kappa B / metabolism
  • Promoter Regions, Genetic
  • Ribonucleases
  • Serum Response Factor / metabolism*
  • Signal Transduction
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcriptional Activation*
  • ets-Domain Protein Elk-1 / metabolism*

Substances

  • Interleukin-1beta
  • NF-kappa B
  • Serum Response Factor
  • Transcription Factors
  • ets-Domain Protein Elk-1
  • Ribonucleases
  • ZC3H12A protein, human