10-Hydroxy-2-decenoic acid from Royal jelly: a potential medicine for RA

J Ethnopharmacol. 2010 Mar 24;128(2):314-21. doi: 10.1016/j.jep.2010.01.055. Epub 2010 Feb 4.


Aim of the study: Rheumatoid arthritis synovial fibroblasts (RASFs) are known to produce matrix metalloproteinases (MMPs) and cause joint destruction. The purpose of this study is to develop a potential medicine for rheumatoid arthritis (RA).

Materials and methods: To this end, first, the MMPs inhibition factor was purified from an alkali-solubilized fraction of RJ (Apis mellifera) by C18 reverse-phase column chromatography and identified as 10-hydroxy-2-decenoic acid (10H2DA) by LTQ XL analysis. Next, Experimental test 10H2DA how to inhibited the activities of MMPs: with RASFs isolated from rheumatoid tissues by enzymatic digestion, cultures in monolayers were treated with 10H2DA (0.5mM, 1mM, and 2mM) or PBS for 2h followed by stimulation with TNF-alpha (10 ng/ml) for 2h, mRNA. Protein levels of MMP-1 and MMP-3 were measured by real-time PCR and enzyme-linked immunosorbent assay (ELISA), the DNA-binding activity of activator protein-1 (AP-1) and nuclear factor kappaB (NF-kappaB) by electrophoretic mobility shift assay (EMSA), and the protein kinase activity of p38, ERK and JNK by kinase assay.

Results: The molecular investigation revealed that the 10H2DA-mediated suppression was likely to occur through blocking p38 kinase and c-Jun N-terminal kinase-AP-1 signaling pathways. In contrast, 10H2DA had no effect on extracellular signal-regulated kinase activity, NF-kappaB DNA-binding activity and IkappaBalpha degradation.

Conclusion: These results suggest that 10H2DA may be of potential therapeutic value in inhibiting joint destruction in RA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antirheumatic Agents / pharmacology*
  • Arthritis, Rheumatoid / enzymology*
  • Arthritis, Rheumatoid / metabolism
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Fatty Acids / chemistry*
  • Fatty Acids, Monounsaturated / chemistry
  • Fatty Acids, Monounsaturated / pharmacology*
  • Fibroblasts / drug effects
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Humans
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Matrix Metalloproteinase 1 / metabolism
  • Matrix Metalloproteinase 3 / metabolism
  • Matrix Metalloproteinases / metabolism
  • NF-kappa B / metabolism
  • RNA, Messenger / metabolism
  • Signal Transduction / drug effects*
  • Transcription Factor AP-1 / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology


  • Antirheumatic Agents
  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • NF-kappa B
  • RNA, Messenger
  • Transcription Factor AP-1
  • Tumor Necrosis Factor-alpha
  • 2-decenoic acid
  • 10-hydroxy-2-decenoic acid
  • JNK Mitogen-Activated Protein Kinases
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 1
  • royal jelly