Abstract
Oxysterols activating liver X receptors (LXRs) repress expression of pro-inflammatory genes and have anti-inflammatory effects. Here, we show for the first time that bone marrow-derived murine mast cells (BMMCs) predominantly express LXRbeta. 25-hydroxycholesterol, a representative LXR activating oxysterol, suppressed IL-6 production and degranulation response in BMMCs following engagement of high-affinity IgE receptor (FcepsilonRI). Interestingly, 25-hydroxycholesterol reduced cell-surface FcepsilonRI expression by inhibiting assembly of FcepsilonRIalpha and FcepsilonRIbeta. We demonstrate that LXR activation was involved in the suppression of IL-6 production in BMMCs, but that reduced FcepsilonRI expression and degranulation response was mediated in an LXR-independent manner.
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibody Affinity / drug effects
-
Bone Marrow Cells / drug effects
-
Bone Marrow Cells / immunology
-
Bone Marrow Cells / metabolism
-
Bone Marrow Cells / physiology
-
Cell Degranulation / drug effects
-
Cells, Cultured
-
Cholestanols / pharmacology
-
Down-Regulation / drug effects
-
Hydroxycholesterols / pharmacology
-
Interleukin-6 / metabolism
-
Liver X Receptors
-
Mast Cells / drug effects*
-
Mast Cells / immunology
-
Mast Cells / metabolism
-
Mast Cells / physiology
-
Mice
-
Mice, Inbred C57BL
-
Orphan Nuclear Receptors / agonists
-
Orphan Nuclear Receptors / metabolism
-
Orphan Nuclear Receptors / physiology*
-
Receptors, IgE / metabolism
-
Receptors, IgE / physiology*
-
Signal Transduction / drug effects
-
Signal Transduction / physiology
-
Sterols / pharmacology*
Substances
-
Cholestanols
-
Hydroxycholesterols
-
Interleukin-6
-
LY 295427
-
Liver X Receptors
-
Orphan Nuclear Receptors
-
Receptors, IgE
-
Sterols
-
25-hydroxycholesterol