Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1

Biosci Biotechnol Biochem. 2010;74(2):430-2. doi: 10.1271/bbb.90692. Epub 2010 Feb 7.

Abstract

A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 degrees C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.

MeSH terms

  • Cloning, Molecular
  • Genes, Plant
  • Oryza / genetics
  • Oryza / metabolism*
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification*
  • Plant Proteins / metabolism*
  • Ubiquitin / genetics
  • Ubiquitin / isolation & purification*
  • Ubiquitin / metabolism*

Substances

  • Plant Proteins
  • Ubiquitin