Antihyperglycaemic effect of an unusual amino acid (4-hydroxyisoleucine) in C57BL/KsJ-db/db mice

Nat Prod Res. 2010 Feb;24(3):258-65. doi: 10.1080/14786410902836693.


The present report confirms the anti-hyperglycaemic and anti-dyslipidemic properties of 4-hydroxyisoleucine, an unusual amino acid isolated from Trigonella foenum-graecum seeds, for the first time in a well-characterised model of type II diabetes, i.e. db/db mice. 4-Hydroxyisoleucine, when given orally to these mice at 50 mg kg(-1) dose level, significantly (p < 0.05) declined their elevated blood glucose, plasma insulin, triglycerides, total cholesterol, low-density lipoprotein-cholesterol levels and raised their declined plasma high-density lipoprotein-cholesterol level. These results indicate that 4-hydroxyisoleucine exhibits significant potential as an anti-diabetic agent by suppressing progression of type II diabetic states that is suggested by enhancement of insulin sensitivity and glucose uptake in peripheral tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose
  • Body Weight
  • Diabetes Mellitus / drug therapy*
  • Hypoglycemic Agents / chemistry
  • Hypoglycemic Agents / pharmacology*
  • Isoleucine / analogs & derivatives*
  • Isoleucine / chemistry
  • Isoleucine / pharmacology
  • Mice
  • Mice, Inbred Strains
  • Molecular Structure
  • Seeds / chemistry
  • Trigonella / chemistry


  • 4-hydroxyisoleucine
  • Blood Glucose
  • Hypoglycemic Agents
  • Isoleucine