Measurement of sex steroids in murine blood and reproductive tissues by liquid chromatography-tandem mass spectrometry

J Steroid Biochem Mol Biol. 2010 Aug;121(3-5):611-8. doi: 10.1016/j.jsbmb.2010.02.001. Epub 2010 Feb 6.


Accurate measurement of sex steroids is essential to evaluate mouse models for human reproductive development and disorders. The recent advent of liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays that match the sensitivity of steroid immunoassay could overcome problems arising from the limited specificity of steroid immunoassay. In this current study we validate a LC-MS/MS assay for the measurement of key sex steroids from murine serum and reproductive tissues. The assay gave excellent dilutional linearity (r(2)> or =0.98) and reproducibility (CV< or =10% of replicate samples) in serum and reproductive tissues with sensitive quantitation limits; testosterone (T; 2pg), dihydrotestosterone (DHT; 10pg), 5alpha-androstane-3alpha,17beta-diol (3alphaDiol; 40pg), 5alpha-androstane-3beta,17beta-diol (3betaDiol; 40pg), estradiol (E2; 0.5pg) and estrone (E1; 0.3pg). Using 0.1mL sample, T was the only consistently detectable steroid (detection limit 20pg/ml) in both male and female mouse serum. In the testis, T and DHT were quantifiable as were both diols at relatively high levels. Prostatic T levels were low and DHT was determined to be the most abundant androgen in this tissue. Uterine and ovarian levels of E2, E1 and T were measurable, with levels varying according to estrous cycle stage. Hence, we demonstrate that this LC-MS/MS method has the sensitivity, specificity and multi-analyte capability to offer accurate steroid profiling in mouse serum and reproductive tissues.

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Chromatography, Liquid / methods*
  • Female
  • Genitalia, Female / metabolism*
  • Genitalia, Male / metabolism*
  • Gonadal Steroid Hormones / blood
  • Gonadal Steroid Hormones / metabolism*
  • Male
  • Mice
  • Mice, Inbred C3H
  • Reproducibility of Results
  • Steroids / blood
  • Steroids / metabolism*
  • Tandem Mass Spectrometry / methods*


  • Gonadal Steroid Hormones
  • Steroids