Abstract
We have characterized a transgenic mouse line in which enhanced green fluorescent protein (EGFP) is expressed under the control of multimerized LEF-1 responsive elements. In embryos, EGFP was detected in known sites of Wnt activation, including the primitive streak, mesoderm, neural tube, somites, heart, limb buds, mammary placodes, and whisker follicles. In vitro cultured transgenic embryonic fibroblasts upregulated EGFP expression in response to activation of Wnt signaling by GSK3beta inhibition. Mammary tumor cell lines derived from female LEF-EGFP transgenic mice treated with the carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) also express EGFP. Thus, this transgenic line is useful for ex vivo and in vitro studies of Wnt signaling in development and cancer.
(c) 2010 Wiley-Liss, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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9,10-Dimethyl-1,2-benzanthracene
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Animals
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Carcinogens
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Cells, Cultured
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Embryo, Mammalian / cytology
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Embryo, Mammalian / embryology
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Embryo, Mammalian / metabolism*
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Female
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Fibroblasts / cytology
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Fibroblasts / metabolism
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Glycogen Synthase Kinase 3 / metabolism
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Glycogen Synthase Kinase 3 beta
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Immunoblotting
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Lymphoid Enhancer-Binding Factor 1 / genetics*
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Male
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Mammary Neoplasms, Experimental / chemically induced
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Mammary Neoplasms, Experimental / genetics
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Mammary Neoplasms, Experimental / metabolism*
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Mice
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Mice, Transgenic
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Microscopy, Fluorescence
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Response Elements / genetics
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Tumor Cells, Cultured
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Wnt Proteins / metabolism*
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beta Catenin / metabolism
Substances
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Carcinogens
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Lef1 protein, mouse
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Lymphoid Enhancer-Binding Factor 1
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Wnt Proteins
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beta Catenin
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Green Fluorescent Proteins
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9,10-Dimethyl-1,2-benzanthracene
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Glycogen Synthase Kinase 3 beta
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Gsk3b protein, mouse
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Glycogen Synthase Kinase 3