Proteomic analysis of formalin-fixed celloidin-embedded whole cochlear and laser microdissected spiral ganglion tissues

Acta Otolaryngol. 2010 Sep;130(9):984-9. doi: 10.3109/00016481003591749.

Abstract

Conclusion: The results of this study demonstrate that proteomic analysis can be successfully performed on formalin-fixed celloidin-embedded (FFCE) archival human cochlear tissues.

Objective: To investigate the feasibility of analyzing protein expression in archival cochlear tissues.

Material and methods: A new methodology, referred to as Liquid Tissue(TM), was used to extract proteins from human cochlear tissue sections and spiral ganglion tissue isolated by laser microdissection (LMD). Protein identification was performed by bioinformatic analysis of high resolution tandem mass spectrometric data from fractionated tryptic peptide samples.

Results: Twenty-six proteins were identified with a minimum of 2 unique peptides and 450 proteins were identified with 1 unique peptide at a confidence level of 95% in cochlear tissue. Ten proteins were identified with a minimum of 2 unique peptides and 485 proteins were identified with 1 unique peptide at a confidence level of 95% in spiral ganglion tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fixatives
  • Formaldehyde
  • Humans
  • Lasers
  • Microdissection
  • Proteomics*
  • Spiral Ganglion / metabolism*
  • Tissue Fixation

Substances

  • Fixatives
  • Formaldehyde