Nutritional effects of culture media on mycoplasma cell size and removal by filtration

Biologicals. 2010 Mar;38(2):214-7. doi: 10.1016/j.biologicals.2009.11.001. Epub 2010 Feb 9.

Abstract

Careful media filtration prior to use is an important part of a mycoplasma contamination prevention program. This study was conducted to increase our knowledge of factors that influence efficient filtration of mycoplasma. The cell size of Acholeplasma laidlawii was measured after culture in various nutritional conditions using scanning electron microscopy. The maximum cell size changed, but the minimum cell size remained virtually unchanged and all tested nutritional conditions resulted in a population of cells smaller than 0.2 microm. Culture in Tryptic Soy Broth (TSB) resulted in an apparent increase in the percentage of very small cells which was not reflected in increased penetration of non-retentive 0.2 microm rated filters. A. laidlawii cultured in selected media formulations was used to challenge 0.2 microm rated filters using mycoplasma broth base as the carrier fluid. We used 0.2 microm rated filters as an analytical tool because A. laidlawii is known to penetrate 0.2 microm filters and the degrees of penetration can be compared. Culture of A. laidlawii in TSB resulted in cells that did not penetrate 0.2 microm rated filters to the same degree as cells cultured in other media such as mycoplasma broth or in TSB supplemented with 10% horse serum.

Publication types

  • Evaluation Study

MeSH terms

  • Acholeplasma laidlawii / cytology
  • Acholeplasma laidlawii / drug effects
  • Acholeplasma laidlawii / growth & development
  • Acholeplasma laidlawii / physiology
  • Bacteriological Techniques / methods
  • Colony Count, Microbial
  • Culture Media / analysis
  • Culture Media / pharmacology*
  • Filtration / methods*
  • Membranes, Artificial
  • Micropore Filters
  • Mycoplasma / drug effects
  • Mycoplasma / growth & development*
  • Mycoplasma / isolation & purification*
  • Mycoplasma / physiology
  • Nutritional Physiological Phenomena / drug effects*
  • Particle Size
  • Sterilization / methods

Substances

  • Culture Media
  • Membranes, Artificial