GammaH2AX as a molecular marker of aging and disease

Epigenetics. 2010 Feb 16;5(2):129-36. doi: 10.4161/epi.5.2.11080. Epub 2010 Mar 1.


Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form gammaH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete gammaH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of gammaH2AX as a molecular marker of aging and disease is analysed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aging / metabolism*
  • Aging / pathology
  • Animals
  • Biomarkers / metabolism
  • DNA Damage
  • Disease*
  • Histones / metabolism*
  • Humans
  • Inflammation / pathology


  • Biomarkers
  • Histones