Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain

EMBO J. 2010 Mar 17;29(6):1033-44. doi: 10.1038/emboj.2010.5. Epub 2010 Feb 11.


During clathrin-mediated endocytosis, adaptor proteins play central roles in coordinating the assembly of clathrin coats and cargo selection. Here we characterize the binding of the yeast endocytic adaptor Sla1p to clathrin through a variant clathrin-binding motif that is negatively regulated by the Sla1p SHD2 domain. The crystal structure of SHD2 identifies the domain as a sterile alpha-motif (SAM) domain and shows a propensity to oligomerize. By co-immunoprecipitation, Sla1p binds to clathrin and self-associates in vivo. Mutations in the clathrin-binding motif that abolish clathrin binding and structure-based mutations in SHD2 that impede self-association result in endocytosis defects and altered dynamics of Sla1p assembly at the sites of endocytosis. These results define a novel mechanism for negative regulation of clathrin binding by an adaptor and suggest a role for SAM domains in clathrin-mediated endocytosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Vesicular Transport / chemistry
  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Binding Sites
  • Clathrin / chemistry
  • Clathrin / metabolism*
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / metabolism
  • Endocytosis / physiology*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism


  • Adaptor Proteins, Vesicular Transport
  • Clathrin
  • Cytoskeletal Proteins
  • SLA1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins

Associated data

  • PDB/3IDW