The relationship between preoperative clinical scores and immunohistological evaluation of surgically resected tissues in chronic severe ocular surface diseases

Jpn J Ophthalmol. 2010 Jan;54(1):66-73. doi: 10.1007/s10384-009-0770-8. Epub 2010 Feb 12.


Purpose: To clarify the relationship between clinical symptoms and histological status in patients with ocular cicatricial pemphigoid (OCP) and Stevens-Johnson syndrome (SJS).

Methods: Clinical symptoms of four OCP and eight SJS patients in the chronic phase were scored with our recently proposed grading system. The histological status of the pannus tissue removed from the corneal surface during surgery was investigated using immunohistological techniques.

Results: All participants showed total loss of the palisades of Vogt and conjunctivalization of the entire corneal surface. All pannus tissues expressed the conjunctival epithelium marker CK4/13. The pannus tissue in clinically keratinized SJS expressed skin epidermal major cytokeratins, but the tissues of nonkeratinized SJS did not.

Conclusions: Clinical observation and the use of our recently proposed grading system agreed with the immunohistological status with respect to keratinization, cell proliferation, and corneal/conjunctival cell typing. These findings facilitate our understanding of the pathogenesis of OCP and SJS, and will hopefully contribute to the development of future treatment strategies and improve predictions of the postoperative prognosis of ocular surface reconstruction in patients with OCP and SJS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, CD / metabolism
  • Biomarkers / metabolism
  • Cell Proliferation
  • Chronic Disease
  • Conjunctiva / metabolism
  • Conjunctiva / pathology*
  • Conjunctival Diseases / diagnosis*
  • Conjunctival Diseases / metabolism
  • Conjunctival Diseases / surgery
  • Cornea / metabolism
  • Cornea / pathology*
  • Corneal Diseases / diagnosis*
  • Corneal Diseases / metabolism
  • Corneal Diseases / surgery
  • Epithelial Cells / pathology
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Keratins / metabolism
  • Ki-67 Antigen
  • Male
  • Middle Aged
  • Pemphigoid, Benign Mucous Membrane / diagnosis*
  • Pemphigoid, Benign Mucous Membrane / metabolism
  • Pemphigoid, Benign Mucous Membrane / surgery
  • Preoperative Period
  • Stem Cells / pathology
  • Stevens-Johnson Syndrome / diagnosis*
  • Stevens-Johnson Syndrome / metabolism
  • Stevens-Johnson Syndrome / surgery
  • Visual Acuity
  • Young Adult


  • Antigens, CD
  • Biomarkers
  • Ki-67 Antigen
  • Keratins