Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma

Leuk Res. 2010 Jul;34(7):932-8. doi: 10.1016/j.leukres.2010.01.021. Epub 2010 Feb 11.


Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79alphacy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gammadelta (TCR-gammadelta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin's lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / analysis
  • Antigens, Neoplasm / analysis
  • B-Lymphocytes / chemistry
  • B-Lymphocytes / pathology*
  • Biopsy, Fine-Needle
  • Cell Line, Tumor / chemistry
  • Cell Line, Tumor / pathology*
  • Cell Separation
  • Clone Cells / chemistry
  • Clone Cells / pathology
  • Dog Diseases / pathology*
  • Dogs
  • Flow Cytometry
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Immunophenotyping
  • Lymph Nodes / pathology
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Lymphoma, Large B-Cell, Diffuse / veterinary*
  • Male
  • Neoplasm Proteins / analysis
  • Receptors, Antigen, T-Cell, gamma-delta / analysis


  • Antigens, CD
  • Antigens, Neoplasm
  • Neoplasm Proteins
  • Receptors, Antigen, T-Cell, gamma-delta