Nonmuscle myosin II localization is regulated by JNK during Drosophila larval wound healing

Biochem Biophys Res Commun. 2010 Mar 19;393(4):656-61. doi: 10.1016/j.bbrc.2010.02.047. Epub 2010 Feb 12.

Abstract

We investigated cell shape changes during wound closure in the Drosophila larval epidermis. During reepithelialization, epidermal cells permanently change shape from pentagonal or hexagonal to irregular forms. This process requires zipper, a gene encoding the Drosophila nonmuscle myosin II heavy chain. Following wounding, myosin II is localized at the wound margin and at the rear end of individual cells located within several rows from the wound hole. The c-Jun N-terminal kinase (JNK) pathway is essential for this myosin II localization. These results suggest that not only the wound leading edge but also the cells lying distal to the leading edge cells actively participate in epithelial cell sheet migration during wound hole closure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Shape
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / metabolism
  • Drosophila melanogaster / physiology*
  • Epidermis / metabolism
  • Epidermis / physiology*
  • JNK Mitogen-Activated Protein Kinases / genetics
  • JNK Mitogen-Activated Protein Kinases / metabolism*
  • Larva / metabolism
  • Larva / physiology
  • Membrane Proteins / metabolism*
  • Myosin Heavy Chains / metabolism*
  • Wound Healing*

Substances

  • Drosophila Proteins
  • Membrane Proteins
  • Zip protein, Drosophila
  • JNK Mitogen-Activated Protein Kinases
  • Myosin Heavy Chains