Parvovirus B19 infection associated with Hashimoto's thyroiditis in adults

J Infect. 2010 May;60(5):360-70. doi: 10.1016/j.jinf.2010.02.006. Epub 2010 Feb 12.


Objective: Parvovirus B19 is a common human pathogen, which has been linked to autoimmune diseases recently. The aim of the study is to evaluate whether B19 is involved in adult Hashimoto's thyroiditis (HT).

Methods: Eighty-six thyroid tissues from the adult patients with a spectrum of thyroid disorders were examined for B19 DNA and capsid protein by nested PCR, in-situ hybridization and immunohistochemistry. The presence of viral DNA in HT epithelium was studied by laser-capture microdissection and sequencing of PCR products. The expressions of nuclear factor-kappaB (NF-kappaB) and interleukin-6 were investigated by immunohistochemistry.

Results: B19 DNA was significantly present in HT tissues by both PCR (29/32, 90.6%) and in-situ hybridization (23/32, 71.9%, all p < 0.01) compared with normal thyroid tissue (7/16, 43.8%; 2/16, 12.5%). Laser-capture microdissection further confirmed this difference. B19 capsid protein in HT group was significantly higher than that in all the control groups (p < 0.01), and the expression of NF-kappaB and interleukin-6 in HT tissues was up-regulated. NF-kappaB was well co-localized with B19 protein in thyroid epithelia by double-labeling immunofluorescence and confocal microscopy.

Conclusions: The presence of B19 nuclear acid and viral protein was significantly common in HT tissues and it suggested a possible role of B19 in adult HT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Capsid Proteins / genetics
  • DNA, Viral / genetics
  • Female
  • Gene Expression
  • Hashimoto Disease / complications*
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Interleukin-6 / biosynthesis
  • Male
  • Microscopy, Confocal
  • Middle Aged
  • NF-kappa B / biosynthesis
  • Parvoviridae Infections / epidemiology*
  • Parvoviridae Infections / virology*
  • Parvovirus B19, Human / isolation & purification*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Up-Regulation
  • Young Adult


  • Capsid Proteins
  • DNA, Viral
  • Interleukin-6
  • NF-kappa B