Organelle-specific, rapid induction of molecular activities and membrane tethering

Nat Methods. 2010 Mar;7(3):206-8. doi: 10.1038/nmeth.1428. Epub 2010 Feb 14.


Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Membrane / metabolism*
  • Dimerization
  • Endoplasmic Reticulum / metabolism*
  • Golgi Apparatus / metabolism
  • HeLa Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins / physiology
  • Mice
  • Mitochondria / metabolism*
  • NIH 3T3 Cells
  • Protein-Serine-Threonine Kinases / physiology
  • TOR Serine-Threonine Kinases
  • Tacrolimus Binding Proteins / metabolism*


  • Intracellular Signaling Peptides and Proteins
  • MTOR protein, human
  • TOR Serine-Threonine Kinases
  • mTOR protein, mouse
  • Protein-Serine-Threonine Kinases
  • Tacrolimus Binding Proteins