Extraction of RNA from fermented milk products for in situ gene expression analysis

Anal Biochem. 2010 May 15;400(2):307-9. doi: 10.1016/j.ab.2010.02.010. Epub 2010 Feb 13.


This work describes a simple method for extracting high-quality microbial RNA from fermented milk. Pretreatment consists merely of adding a concentrated solution of sodium citrate to the milk product. The proposed method provides consistently better yields of high-quality RNA than the conventional method independent of the texture of the fermented milk. Furthermore, it can be used with small amounts of starting material, requiring only the use of Eppendorf-size centrifuges-a great advantage for analytical laboratories. The RNA obtained is suitable for the detection of live microorganisms and for transcriptional studies based on quantitative reverse transcription polymerase chain reaction (RT-qPCR).

Publication types

  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Animals
  • Cattle
  • Citrates / chemistry
  • Cultured Milk Products / microbiology*
  • RNA, Bacterial / chemistry*
  • RNA, Bacterial / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium Citrate
  • Transcription, Genetic


  • Citrates
  • RNA, Bacterial
  • Sodium Citrate