Transcriptional regulation of the rice arginine decarboxylase (Adc1) and S-adenosylmethionine decarboxylase (Samdc) genes by methyl jasmonate

Plant Physiol Biochem. 2010 Jul;48(7):553-9. doi: 10.1016/j.plaphy.2010.01.014. Epub 2010 Jan 28.

Abstract

We investigated the effect of methyl jasmonate (MeJa) treatment on the expression of two genes in the rice polyamine biosynthesis pathway and on the polyamine content in wild type plants and transgenic rice plants expressing a Datura stramonium (Ds) Adc cDNA, the latter accumulating up to three-fold the normal level of putrescine. Exogenous MeJa transiently inhibited the expression of OsAdc1, OsSamdc and Spermidine synthase (OsSpds) genes in the polyamine biosynthesis pathway, probably through transcriptional repression. There was also a similar negative impact on the DsAdc transgene in transgenic plants, even though a constitutive promoter was used to drive transgene expression. The free putrescine content was reduced significantly in the leaves of both wild type and transgenic plants in response to MeJa, although the magnitude of the effect was greater in wild type plants. We discuss our findings with respect to the previously proposed threshold model of polyamine metabolism in plants subjected to abiotic stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / metabolism*
  • Acetates / pharmacology
  • Adenosylmethionine Decarboxylase / genetics*
  • Adenosylmethionine Decarboxylase / metabolism
  • Carboxy-Lyases / genetics*
  • Carboxy-Lyases / metabolism
  • Cyclopentanes / metabolism*
  • Cyclopentanes / pharmacology
  • DNA, Complementary
  • Datura / genetics
  • Gene Expression / drug effects
  • Gene Expression Regulation, Plant / drug effects*
  • Genes, Plant*
  • Oryza / genetics
  • Oryza / metabolism*
  • Oxylipins / metabolism*
  • Oxylipins / pharmacology
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology
  • Plant Leaves
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Polyamines / metabolism*
  • Promoter Regions, Genetic
  • Putrescine / metabolism
  • Spermidine / metabolism
  • Transgenes

Substances

  • Acetates
  • Cyclopentanes
  • DNA, Complementary
  • Oxylipins
  • Plant Growth Regulators
  • Plant Proteins
  • Polyamines
  • methyl jasmonate
  • Carboxy-Lyases
  • arginine decarboxylase
  • Adenosylmethionine Decarboxylase
  • Spermidine
  • Putrescine