Cytoskeleton in TFG-beta- and bFGF-modulated endothelial monolayer repair

Exp Cell Res. 1991 May;194(1):42-7. doi: 10.1016/0014-4827(91)90127-g.

Abstract

Endothelial cell proliferation and migration in vitro is depressed by transforming growth factor beta (TFG-beta) and enhanced by basic fibroblast growth factor (bFGF) treatment. This study examines interactions between cytoskeletal changes and cell proliferation in regenerating endothelial monolayers treated with bFGF, TFG-beta, and both factors. As previously described by others, monolayer regeneration is enhanced by bFGF and reduced by TFG-beta. Endothelial cell morphology is altered by TFG-beta treatment. Cells lose their cobblestone appearance and assume a pleomorphic shape. Actin microfilament staining is modified in both intact and regenerating TFG-beta-treated monolayers as well. There is a loss of dense peripheral band staining and an enhancement in staining intensity of cytoplasmic stress fibers. No such alterations are seen in bFGF-treated cultures. Cell proliferation at the wound edge, as indicated by bromodeoxyuridine incorporation, is inhibited by TGF-beta. Although monolayer repair is modulated by growth factor treatment, centrosome reorientation and microtubule staining patterns are not altered by either factor. Thus these factors appear to have effects on a mechanism(s) other than centrosome reorientation which may be involved in repair of denuded endothelial monolayers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cell Movement / drug effects
  • Cell Movement / physiology
  • Cells, Cultured
  • Cytoskeleton / drug effects
  • Cytoskeleton / physiology
  • Cytoskeleton / ultrastructure*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / ultrastructure
  • Fibroblast Growth Factor 2 / physiology*
  • Fluorescent Antibody Technique
  • Microtubules / drug effects
  • Microtubules / physiology
  • Microtubules / ultrastructure
  • Transforming Growth Factor beta / physiology*

Substances

  • Transforming Growth Factor beta
  • Fibroblast Growth Factor 2