Nebulin as a giant actin-binding template protein in skeletal muscle sarcomere. Interaction of actin and cloned human nebulin fragments

FEBS Lett. 1991 Apr 9;281(1-2):93-6. doi: 10.1016/0014-5793(91)80366-b.

Abstract

Nebulin is a family of giant sarcomere matrix proteins of 600-900 kDa in most vertebrate skeletal muscles. Recent sequence analysis suggests that human nebulin is mainly composed of a large number (greater than 200) of conserved repeats of approximately 35 residues. Two cloned nebulin fragments, consisting of 6 and 8 of the repeats, have been expressed in E. coli using the pET3d vector. Both F-actin cosedimentation and solid-phase binding assays demonstrated a specific binding of these nebulin fragments to actin. This finding suggests that nebulin is a giant protein which binds actin at multiple sites in a template-manner. The presence of an actin-binding template protein in the skeletal muscle sarcomere may have significant implications in the assembly and function of the contractile apparatus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Humans
  • Kinetics
  • Molecular Weight
  • Muscle Proteins / genetics
  • Muscle Proteins / isolation & purification
  • Muscle Proteins / metabolism*
  • Muscles / metabolism*
  • Plasmids
  • Protein Binding
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sarcomeres / metabolism*

Substances

  • Actins
  • Muscle Proteins
  • Recombinant Proteins
  • nebulin