Abstract
Hydroxyapatite (HA) is a widely used calcium phosphate implant substitute and has dissolution property. Although HA has been shown a beneficial effect on osteoblast differentiation, the exact mechanism is still unclear. In the present study, we proposed that Ca(2+) released from HA activated the expression bone associated proteins, OPN and BSP, mediated by L-type calcium channel and calcium/calmodulin-dependent protein kinase (CaMK) 2 which resulted into improved osteoblast differentiation. Results showed that HA elevated ALP expression as well as OPN and BSP expression in MC3T3-E1 cells. The result from western blot of CaMK2alpha indicated that HA released Ca(2+) activated CaMK2 through L-type calcium channel. Furthermore, upregulation of OPN and BSP mRNA expression was significantly inhibited when blocking both the L-type calcium channel and CaMK2. These findings suggested that HA accelerated the osteoblast differentiation by releasing Ca(2+).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoimmune Lymphoproliferative Syndrome
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Bone and Bones / metabolism
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Calcium / metabolism
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Calcium / pharmacology*
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Calcium Channels, L-Type / drug effects
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Calcium Channels, L-Type / genetics
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Calcium Channels, L-Type / metabolism
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Calcium Metabolism Disorders / genetics
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Calcium Metabolism Disorders / metabolism
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Calcium Phosphates / metabolism
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Calcium Phosphates / pharmacology
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Calcium, Dietary / metabolism
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Calcium, Dietary / pharmacology
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Cell Differentiation / drug effects*
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Cell Differentiation / genetics
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Clone Cells
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Durapatite / metabolism
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Durapatite / pharmacology
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Integrin-Binding Sialoprotein
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Ions / metabolism
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Ions / pharmacology
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Mice
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Osteoblasts* / cytology
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Osteoblasts* / drug effects
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Osteoblasts* / metabolism
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Osteogenesis / genetics
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Sialoglycoproteins
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Up-Regulation
Substances
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Calcium Channels, L-Type
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Calcium Phosphates
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Calcium, Dietary
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Ibsp protein, mouse
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Integrin-Binding Sialoprotein
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Ions
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Sialoglycoproteins
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Durapatite
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calcium phosphate
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Calcium