Maturation of dendritic cell precursors into IL12-producing DCs by J-LEAPS immunogens

Cell Immunol. 2010;262(1):1-5. doi: 10.1016/j.cellimm.2010.01.003. Epub 2010 Feb 1.

Abstract

LEAPS (ligand epitope antigen presentation system) vaccines consist of a peptide containing a major histocompatibility antigen binding peptide conjugated to an immune cell binding ligand (ICBL) such as the 'J' peptide from beta-2-microglobulin. Treatment of monocytes, monocytes plus GMCSF, or monocytes plus GMCSF and IL4 with JgD (containing a peptide from gD of herpes simplex virus type 1) or JH (with a peptide from HIV p17 gag protein) was sufficient to promote their maturation into Interleukin 12 producing dendritic cells. JgD-dendritic cells supported allotypic activation of T cells to produce Th1-related cytokines.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antigen Presentation*
  • Cell Differentiation*
  • Coculture Techniques
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology*
  • Epitopes / immunology*
  • Humans
  • Immunoglobulin J-Chains / immunology*
  • Interleukin-12 / biosynthesis*
  • Interleukin-12 / immunology

Substances

  • Epitopes
  • Immunoglobulin J-Chains
  • Interleukin-12